BIOTECHNOLOGY

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BIOT 1113 - Introduction to Biotechnology
An introduction to biotechnology including career exploration, history and applications of DNA/RNA technology, molecular biology bioethics, safe laboratory practices and bioinformatics. The course covers calculations for solution preparations, instruction on making buffers, determining pH, pipetting, making dilutions, calibration of pipets and balances as well as an introduction into DNA and protein isolation and analysis.

BIOT 1315 - Biotechnology Laboratory Methods and Techniques
Emphasis is on laboratory operations, management, equipment, instrumentation, quality control techniques, and safety procedures. Lecture emphasis will be on biochemistry, bioorganic topics and the scientific method, including assay design. Laboratory practice will stress lab safety and the design of assays for various classes of biochemicals, including RNA, DNA, enzyme and total protein assays. Prerequisites: BIOT 1113 or concurrent enrollment, BIOL 1224 or concurrent enrollment, and CHEM 1114 or CHEM 1315 all with grades of “C” or better.

BIOT 1534 - Cell Culture Techniques
This course will discuss the lecture topics of the cell cycle, cell requirements for growth, and bacterial and animal viruses. Lab topics will include sterile technique. In the study of eukaryotic cell culture techniques, the laboratory emphasizes the principles and practices of media preparation and sterilization, initiation, cultivation, maintenance, and the preservation of cell, including applications such as project development and biomanufacturing. The topic of lab safety with potentially biohazardous agents will be covered. Prerequisites: BIOT 1315 or concurrent enrollment and BIOL 2164 or concurrent enrollment.

BIOT 2101 - Biotechnology Quality Assurance
The issues of quality control in a biotechnology setting will be explored, along with FDA, EPA and OSHA regulations. Prerequisite: BIOT 1113.

BIOT 2246 - Molecular Biology and Techniques
An introduction to the principles of heredity and theory of molecular biology with an emphasis on DNA replication, transcription, gene expression and regulation, recombinant DNA, and RNA interference. Techniques such as DNA and RNA isolation, DNA restriction digests, cell transformation, transfection, PCR, DNA sequencing, real-time PCR, blot assays, microarray analysis, DNA mapping, cloning and DNA bioinformatic tools will be discussed and explored. Prerequisites: BIOT 1315, BIOL 2164 and CHEM 1124 or CHEM 1415 with grades of “C” or better.

BIOT 2335 - Biotechnology Proteomics and Instrumentation
Lecture will discuss theory, applications, and operation of various analytical instruments with an emphasis on protein purification in both an R&D laboratory and a biomanufacturing facility. Laboratory exercises will focus on calibration of instruments, quantitative and qualitative analysis by chromatography, centrifugation, spectrophotometry, and SDS-PAGE gel analysis. Additionally, basic immunology including humoral and cellular immunity with ELISAs, Western Blots and cytoimmunology will be taught. An introduction to protein bioinformatics tools is included. Prerequisites: BIOT 1534 with a grade of “C” or better and CHEM 1124 or CHEM 1415 with a grade of “C” or better.

BIOT 2512 - Biotechnology Apprenticeship
This is an experience external to the college for an advanced student in a specialized field involving a written agreement between the educational institution and a business or industry partner. Mentored and supervised by a workplace employee, the student achieves objectives in a biotechnical project that are developed and documented by the college that directly relate to specific occupational outcomes. This may be a paid or unpaid experience. This course may be repeated if topics and learning outcomes vary. The student should have a minimum of 160 hrs on the job. Prerequisites: BIOT 2246 and BIOT 2335 with grades of “C” or better.

BIOT 2990 - Selected Topics in Biotechnology
The study and/or analysis of a selected topic in Biotechnology. May be repeated with a different topic.

The time required for laboratory may vary by course.
Please refer to the course syllabus for more information.

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